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Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae.
- Source :
-
FEBS Journal . Jul2009, Vol. 276 Issue 14, p4234-4248. 15p. 1 Chart, 7 Graphs. - Publication Year :
- 2009
-
Abstract
- Saccharomyces cerevisiae cells control their cell size at a point in late G1 called Start. Here, we describe a negative role for the Sin3/Rpd3 histone deacetylase complex in the regulation of cell size at Start. Initiation of G1/S-specific transcription of CLN1, CLN2 and PCL1 in a sin3Δ strain occurs at a reduced cell size compared with a wild-type strain. In addition, inactivation of the transcriptional regulator SIN3 partially suppressed a cln3Δ mutant, causing sin3Δcln3Δ double mutants to start the cell cycle at wild-type size. Chromatin immunoprecipitation results demonstrate that Sin3 and Rpd3 are recruited to promoters of SBF (Swi4/Swi6)-regulated genes, and reveal that binding of Sin3 to SBF-specific promoters is cell-cycle regulated. We observe that transcriptional repression of SBF-dependent genes in early G1 coincides with the recruitment of Sin3 to specific promoters, whereas binding of Sin3 is abolished from Swi4/Swi6-regulated promoters when transcription is activated at the G1 to S phase transition. We conclude that the Sin3/Rpd3 histone deacetylase complex helps to prevent premature activation of the S phase in daughter cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1742464X
- Volume :
- 276
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- FEBS Journal
- Publication Type :
- Academic Journal
- Accession number :
- 42110316
- Full Text :
- https://doi.org/10.1111/j.1742-4658.2009.07095.x