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The human CD6 gene is transcriptionally regulated by RUNX and Ets transcription factors in T cells
- Source :
-
Molecular Immunology . Jul2009, Vol. 46 Issue 11/12, p2226-2235. 10p. - Publication Year :
- 2009
-
Abstract
- Abstract: CD6 is a lymphocyte surface receptor involved in lymphocyte activation and differentiation processes that is constitutively expressed on developing and mature T cells and on the B1a cells. To define the molecular basis for the tissue-specific expression of CD6 we have identified the transcription factors that control the activity of the proximal regulatory region of the human CD6 gene. The TATA-less CD6 promoter contains multiple transcriptional start sites, and its preferential activity in human T lymphocytes is dependent on RUNX- and Ets-binding sites located within a highly conserved region. RUNX and Ets-1 factors transactivated the CD6 promoter through recognition of the −215 and −230 binding sites, respectively. Chromatin immunoprecipitation assays revealed that RUNX1 constitutively occupies the CD6 promoter in vivo, and knockdown experiments demonstrated that the steady-state level of CD6 mRNA is dependent on the expression of RUNX1, RUNX3 and Ets-1 transcription factors. Therefore, RUNX1/3 and Ets1 control the expression of CD6 in human T lymphocytes, thus expanding the range of T-cell specific and developmentally regulated lymphocyte gene targets involved in T-cell activation and differentiation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 46
- Issue :
- 11/12
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 41582509
- Full Text :
- https://doi.org/10.1016/j.molimm.2009.04.018