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Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family.
- Source :
-
Molecular Ecology Resources . Jan2009, Vol. 9 Issue 1, p405-408. 4p. 2 Charts. - Publication Year :
- 2009
-
Abstract
- We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt ( Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples ( n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy–Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy–Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt ( Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt ( Spirinchus thaleichthys). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1755098X
- Volume :
- 9
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Molecular Ecology Resources
- Publication Type :
- Academic Journal
- Accession number :
- 39361488
- Full Text :
- https://doi.org/10.1111/j.1755-0998.2008.02254.x