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Practical use of chemical probes for reactive oxygen species produced in biological systems by γ-irradiation
- Source :
-
Radiation Physics & Chemistry . May2009, Vol. 78 Issue 5, p323-327. 5p. - Publication Year :
- 2009
-
Abstract
- Abstract: Application of chemical probes, for detection of reactive oxygen species (ROS), was tested during γ-irradiation. The ethanol/α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN) and 3,3′-diaminobenzidine (DAB) were structurally stable enough to detect OH and H2O2, increasingly generated by γ-irradiation up to 1000Gy. Interestingly, the production rate of H2O2, but not OH, during γ-irradiation, was significantly different between in vitro systems of lettuce and spinach. These results suggest that 4-POBN and DAB could be utilized as a semi-quantitative probe to quantify OH and H2O2, produced by γ-irradiation up to 1000Gy. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0969806X
- Volume :
- 78
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Radiation Physics & Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38318739
- Full Text :
- https://doi.org/10.1016/j.radphyschem.2009.03.001