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The pivotal role of p38 and NF-κB signal pathways in the maturation of human monocyte-derived dendritic cells stimulated by streptococcal agent OK-432
- Source :
-
Immunobiology . May2009, Vol. 214 Issue 5, p350-358. 9p. - Publication Year :
- 2009
-
Abstract
- Abstract: OK-432, a streptococcal preparation, has been shown as an effective activator to induce human monocyte-derived dendritic cells maturation. During this process, the activation of Toll-like receptor 4 plays an important role. However, the signaling pathway involved in has not been fully understood. In the present study, we investigated the underlying mechanisms, by which OK-432 induced maturation of human monocyte-derived DCs (MoDCs). We observed that exposure of immature MoDCs to OK-432 activated the p38 MAPK and NF-κB pathway, accompanied up-regulated the surface expression of maturation markers CD80, CD83, CD86 and HLA-DR, increased secretion of TNF-α, IL-12 and chemokine, IP-10. In addition, T cells stimulatory capacity was also enhanced. The maturation of MoDCs stimulated by OK-432 was inhibited by treatment with p38 pathway inhibitor, SB203580, or NF-κB pathway inhibitor, BAY-117082. Whereas, blocking of JNK pathway with SP600125 or ERK pathway with PD98059 did not influence OK-432-induced DCs maturation. Taken together, our data indicated that OK-432-induced DCs maturation was due, at least partly to the activation of p38 and NF-κB pathway. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01712985
- Volume :
- 214
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 37572299
- Full Text :
- https://doi.org/10.1016/j.imbio.2008.10.008