Double helix-PSF enables superresolution 3-D imaging.

The article reports on the double-helix point-spread function PSF method by the researchers at Stanford University in California and the University of Colorado in the 3-D optical imaging of objects smaller than 20 nanometers (nms) defeating the optical diffraction limit by an order of magnitude. As stated, researchers are able to localize single fluorescent molecules within 10 to 20 nms over a large depth of field. In addition, the technique holds promise for improving 3-D imaging even.