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Isolation and purification of flavonoid glucosides from Radix Astragali by high-speed counter-current chromatography
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Mar2009, Vol. 877 Issue 8/9, p697-702. 6p. - Publication Year :
- 2009
-
Abstract
- Abstract: High-speed counter-current chromatography methods, combined with resin chromatography were applied to the separation and purification of flavonoid glycosides from the Chinese medicinal herb, Radix Astragali. Five flavonoid glycosides, namely calycosin-7-O-β-d-glucoside, ononin, (6aR, 11aR)-9,10-dimethoxypterocarpan-3-O-β-d-glucoside, (3R)-2′-hydroxy-3′,4′-dimethoxyisoflavan-7-O-β-d-glucoside and calycosin-7-O-β-d-glucoside-6′′-O-acetate, were obtained. Among them, calycosin-7-O-β-d-glucoside-6′′-O-acetate was preparatively separated from Radix Astragali for the first time. Their structures were identified by ESI–MS, 1H NMR, 13C NMR, and 2D NMR. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 877
- Issue :
- 8/9
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 36899671
- Full Text :
- https://doi.org/10.1016/j.jchromb.2009.01.034