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Immunological properties of 60Co gamma-rays irradiated bothropstoxin-I.
- Source :
-
Journal of Radioanalytical & Nuclear Chemistry . Mar2009, Vol. 279 Issue 3, p817-821. 5p. 1 Black and White Photograph, 1 Chart, 3 Graphs. - Publication Year :
- 2009
-
Abstract
- We investigated the immunological behavior of BTHX-1, before and after irradiation. SDS-PAGE showed that BTHX-1 irradiated in the presence of NaNO3, had its structure preserved. Animals’ plasma immunized with native BTHX-1 had high IgG1 titers. The irradiated protein induced high titers of IgG2b. When the toxin was irradiated with t-butanol, there was a slight decrease in the production of IgG2b. Real-time PCR showed that both the IL-2 as for IL4 was more expression from the cells of the animals immunized with BTHX-1 irradiated. These results indicate that irradiation of proteins leads to significant structural modifications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02365731
- Volume :
- 279
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Radioanalytical & Nuclear Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 36844685
- Full Text :
- https://doi.org/10.1007/s10967-008-7416-z