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InPSR26, a Putative Membrane Protein, Regulates Programmed Cell Death during Petal Senescence in Japanese Morning Glory.

Authors :
Shibuya, Kenichi
Yamada, Tetsuya
Suzuki, Tomoko
Shimizu, Keiichi
Ichimura, Kazuo
Source :
Plant Physiology. Feb2009, Vol. 149 Issue 2, p816-824. 9p. 2 Diagrams, 1 Chart, 5 Graphs.
Publication Year :
2009

Abstract

The onset and progression of petal senescence, which is a type of programmed cell death (PCD), are highly regulated. Genes showing changes m expression during petal senescence in Japanese morning glory (Ipomoea nil) were isolated and examined to elucidate their function in PCD. We show here that a putative membrane protein, InPSR26, regulates progression of PCD during petal senescence in Japanese morning glory. InPSR26 is dominantly expressed in petal limbs and its transcript level increases prior to visible senescence symptoms. Transgenic plants with reduced InPSR26 expression (PSR26r lines) showed accelerated petal wilting, with PCD symptoms including cell collapse, ion and anthocyanin leakage, and DNA degradation accelerated in petals compared to wild-type plants. Transcript levels of autophagy- and PCD-related genes (InATG4, InATG8, InVPE, and InBI-1) were reduced in the petals of PSR26r plants. Autophagy visualized by monodansylcadaverine staining confirmed that autophagy is induced in senescing petal cells of wild-type plants and that the percentage of cells containing monodansylcadaverine-stained structures, most likely autophagosomes, was significantly lower in the petals of PSR26r plants, indicating reduced autophagic activity in the PSR26r plants. These results suggest that InPSR26 acts to delay the progression of PCD during petal senescence, possibly through regulation of the autophagic process. Our data also suggest that autophagy delays PCD in petal senescence. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00320889
Volume :
149
Issue :
2
Database :
Academic Search Index
Journal :
Plant Physiology
Publication Type :
Academic Journal
Accession number :
36594723
Full Text :
https://doi.org/10.1104/pp.108.127415