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Pseudorabies virus glycoprotein B can be used to carry foot and mouth disease antigens in DNA vaccination of pigs
- Source :
-
Antiviral Research . Mar2009, Vol. 81 Issue 3, p217-225. 9p. - Publication Year :
- 2009
-
Abstract
- Abstract: To evaluate the feasibility of using pseudorabies virus (PrV) glycoprotein B (gB) as a carrier of foot and mouth disease virus (FMDV) antigens in DNA immunization, FMDV B- and T-cell epitopes were inserted either between the two B-cell epitopes of the N-term subunit of PrV-gB (BT-PrV-gB–N-term construct) or within the B-cell epitope of the C-term subunit of PrV-gB (BT-PrV-gB–C-term construct). Two animal experiments were performed, each with three injections of plasmids 2 weeks apart, followed by a booster inoculation of peptides corresponding to the FMDV epitopes. Control groups of pigs were injected with plasmids encoding either PrV-gB or FMDV-BT, or with empty-pcDNA3. The results of both assays were combined. Significant titers of FMDV neutralizing antibodies were detected after the peptides boost in groups injected with the BT-PrV-gB–C-term construct. Insignificant amounts were detected in groups injected with the BT-PrV-gB–N-term and FMDV-BT constructs. PBMCs from the BT-PrV-gB–N-term groups, isolated after the peptide boost injection, produced IFN-γ and IL-4 mRNAs in vitro when stimulated with FMDV peptides. This was not observed with the other groups. These results imply that PrV-gB can be used to carry FMDV antigens in a DNA vaccine. [Copyright &y& Elsevier]
- Subjects :
- *EPITOPES
*NUCLEIC acids
*IMMUNOGLOBULINS
*VACCINATION
Subjects
Details
- Language :
- English
- ISSN :
- 01663542
- Volume :
- 81
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Antiviral Research
- Publication Type :
- Academic Journal
- Accession number :
- 36563533
- Full Text :
- https://doi.org/10.1016/j.antiviral.2008.11.005