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Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system
- Source :
-
Developmental & Comparative Immunology . May2009, Vol. 33 Issue 5, p697-708. 12p. - Publication Year :
- 2009
-
Abstract
- Abstract: Rabbits are widely used for vaccine development, and investigations of human infectious and autoimmune diseases such as Systemic Lupus Erythematosus (SLE). For these applications, we cloned, sequenced and expressed rabbit B-cell Activating Factor (BAFF), and localized BAFF in cells and tissues of the rabbit immune system. The rabbit homolog of the human BAFF binding site (miniBR3 peptide) within the BAFF-specific receptor BR3 was synthesized. This 26-residue core domain binds to recombinant rabbit BAFF protein. Flow cytometric analyses using purified recombinant rabbit BAFF combined with real-time PCR findings revealed that BAFF detected on peripheral blood B-cells from normal rabbits is probably complexed to BAFF receptors rather than produced by the B-cells. BAFF was detected in developing appendix of young rabbits by immunohistochemical staining suggesting that BAFF plays a role during the period following birth when rabbit B-cell development and pre-immune antibody repertoire diversification and selection is occurring. [Copyright &y& Elsevier]
- Subjects :
- *IMMUNE system
*AUTOIMMUNE diseases
*B cells
*LABORATORY rabbits
Subjects
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 33
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 36477711
- Full Text :
- https://doi.org/10.1016/j.dci.2008.12.001