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Gene Cloning of α-Methylserine Aldolase from Variovorax paradoxus and Purification and Characterization of the Recombinant Enzyme.

Authors :
Nozaki, Hiroyuki
Kuroda, Shinji
Watanabe, Kunihiko
Yokozeki, Kenzo
Source :
Bioscience, Biotechnology & Biochemistry. Oct2008, Vol. 72 Issue 10, p2580-2588. 9p. 1 Diagram, 1 Chart, 5 Graphs.
Publication Year :
2008

Abstract

The article studies the alpha-methylserine aldolase gene from the NBRC15150, NBRC15149 and AJ110406 strains of Variovorax paradoxus that was expressed and cloned in Escherichia coli. It includes an in-depth analysis of the characterization and purification of the recombinant enzyme, as well as a discussion on the issues' implications for biotechnology.

Details

Language :
English
ISSN :
09168451
Volume :
72
Issue :
10
Database :
Academic Search Index
Journal :
Bioscience, Biotechnology & Biochemistry
Publication Type :
Academic Journal
Accession number :
36394581
Full Text :
https://doi.org/10.1271/bbb.80274