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Concanavalin a Immobilized Monosize and Magnetic Poly(glycidyl Methacrylate) Beads for Use in Yeast Invertase Adsorption.
- Source :
-
Journal of Macromolecular Science: Pure & Applied Chemistry . Mar2009, Vol. 46 Issue 3, p232-239. 8p. 1 Black and White Photograph, 1 Diagram, 1 Chart, 8 Graphs. - Publication Year :
- 2009
-
Abstract
- Concanavalin A (Con A) immobilized monosize and magnetic poly(glycidyl methacrylate)[m-poly(GMA)] beads were investigated for specific adsorption of yeast invertase from aqueous solutions. m-Poly(GMA) beads (1.6 μ m in diameter) were prepared by dispersion polymerization in the presence of Fe3O4 nanopowder. The epoxy groups of m-poly(GMA) beads were opened by base catalyses. Then, Con A was immobilized by covalent binding onto the beads. Con A immobilization amount was 12.5 mg/g. The invertase adsorption capacity of the m-poly(GMA)/Con A beads was 111 mg/g. The maximum invertase adsorption on the m-poly(GMA)/Con A beads was observed at pH 5.5. The optimum activity for both free and adsorbed invertase was observed at 50°C. Vmax values were determined as 330 U/mg and 292 U/mg enzyme, for free and adsorbed invertase, respectively. KM values were found to be the same for free and adsorbed invertase (20 mM). Adsorption of invertase via Con A improved the pH stability of invertase. Thermal stability also increased with adsorption. The adsorbed enzyme activity was found to be quite stable in repeated experiments. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ADSORPTION (Chemistry)
*YEAST
*INVERTASE
*ENZYMES
*CHEMICAL reactions
Subjects
Details
- Language :
- English
- ISSN :
- 10601325
- Volume :
- 46
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Macromolecular Science: Pure & Applied Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 36188876
- Full Text :
- https://doi.org/10.1080/10601320802637086