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Detection and chromatographic removal of lipopolysaccharide in preparations of multifunctional galectins
- Source :
-
Biochemical & Biophysical Research Communications . Jan2009, Vol. 379 Issue 1, p155-159. 5p. - Publication Year :
- 2009
-
Abstract
- Abstract: The functional spectrum of human galectins is currently explored, with a wide range of activities being described. The role of galectin-3 as adhesin for bacteria is based on its strong binding to lipopolysaccharides (LPSs), which brings the possibility of such a contamination in galectin preparations to awareness. This assumption was verified in three independent functional assay systems using polymyxin B as inhibitor of LPS-dependent effects. Moreover, a commercial LPS quantification kit also revealed LPS in galectin preparations. Chromatography was effective in removing LPS, suggesting that such a technique needs to be applied to prevent assigning cellular responses to galectins rather than LPS. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 379
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 36139424
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.12.024