A possible role of progesterone receptor in mouse oocyte in vitro fertilization regulated by norethisterone and its reduced metabolite

Abstract: Background: The contraceptive effect of the progestogen norethisterone (NET) and its main metabolites 5α-NET and 3β,5α-NET has been demonstrated in several species, and most studies have focused on the effects of these compounds in the uterus. We previously reported that 5α-NET inhibits the progesterone (P4)-induced acrosome reaction in pig and mouse spermatozoa and induces severe morphological damage in two-cell fertilized mouse oocytes. Study Design: The main goal of this study was to analyze the possible role of P4 receptor (PR) in the effects of NET and 5α-NET on the oocyte fertilization process. Different steroid treatments were used with or without cumulus-enclosed oocytes. Results: It was demonstrated that NET increases the percentage of fertilized oocytes in the same manner as P4 does, while 5α-NET reduces the percentage of fertilized oocytes. This effect was not reversed by P4 in the same concentrations. Conclusion: A possible molecular mechanism for the effects of 5α-NET may be through a PR localized in the oocyte plasma membrane. [Copyright &y& Elsevier]