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Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples
- Source :
-
Experimental Parasitology . Dec2008, Vol. 120 Issue 4, p353-356. 4p. - Publication Year :
- 2008
-
Abstract
- Abstract: A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00144894
- Volume :
- 120
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Experimental Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 35164520
- Full Text :
- https://doi.org/10.1016/j.exppara.2008.09.004