Marcadores y efecto antitumoral, anti-VIH de las hojas y corteza de Calophyllum brasiliense C, "Lagarto caspi, Santa María, Barí" de las zonas de Satipo y Pucallpa. PERÚ SUDAMÉRICA.

We evaluated the antitumoral and antiviral activity of extracts at 10% (w/v) of leaves and bark of Calophyllum brasiliense Cambess of Satipo and Pucallpa. METHODS: CYTED, Skehan and Simmons techniques were used. RESULTS: In Anemia salina the LC50 was at 70, 5 ppm, with 95% superior and inferior limits of 136.50ppm and 36.4ppm with Probits. An inhibition on tumoral cell lines: U251(CNS Cancer), PC-3(Prostate Cancer), HCT15 (Colon cancer), MCF7(Breast cancer) and K562 (Lymphoid tissue cancer) of 109,42%, 95,67%, 96,16%, 102,68% and 95,69% respectively, with p< 0,01 was observed. In the antimitotic evaluation using Allium cepa, after 6 hours the MI of meristematic cells decreased from 13 to 1,0 at a concentration of 0,5% at 25°C. The antiviral activity on Reverse Transcriptase (RT) of the HIV-1, performed with the Lenti RT Kit, presented a LC50 of 70,2905 ug/mI (limit of 95%: 138.89ug/ml-35.5707ug/ml). We detected: alkaloids, coumarins, lactones, pentacyclic compounds, triterpens, and three compounds were isolated CBHS5, CbHS16, CbHS525354. Structural elucidation of the major components were characterized by RMNH1, RMNC13 and Mass Spectrometry and were Betulin, that has an antiinflammatory effect and betulinic acid, that has antitumoral and anti-HIV effects. DISCUSSION: Betulin and betulinic acid could be implied in the pharmacologic action of the leaves extracts of Satipo, that presented a good antitumoral and antiviral activity of 7 2,05% on RT, as opposed to Nevirapine used in the therapy of patients with AIDS, that presented 71.59%activity. [ABSTRACT FROM AUTHOR]