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Characterisation of the metallo-β-lactamase VIM-6 and its genetic support
- Source :
-
International Journal of Antimicrobial Agents . Nov2008, Vol. 32 Issue 5, p446-449. 4p. - Publication Year :
- 2008
-
Abstract
- Abstract: The purpose of this study was to characterise the metallo-β-lactamase VIM-6 and the genetic environment of the bla VIM-6 gene. The bla VIM-6 gene was cloned into an Escherichia coli expression system and the purified VIM-6 enzyme was obtained. VIM-6 has an isoelectric point of 4.9 and a molecular weight of 28.368Da. VIM-6 hydrolysed all tested penicillins, cephalosporins and carbapenems with the exception of aztreonam. In Pseudomonas putida, the bla VIM-6 gene is the first in a class 1 integron also containing bla OXA-10, aacA4, an open reading frame of unknown function, aadA and qacEΔ1. [Copyright &y& Elsevier]
- Subjects :
- *ENZYMES
*CATALYSTS
*ESCHERICHIA
*ENTEROBACTERIACEAE
Subjects
Details
- Language :
- English
- ISSN :
- 09248579
- Volume :
- 32
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- International Journal of Antimicrobial Agents
- Publication Type :
- Academic Journal
- Accession number :
- 34743812
- Full Text :
- https://doi.org/10.1016/j.ijantimicag.2008.05.017