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Continuous in vitro exposure to low-dose genistein induces genomic instability in breast epithelial cells
- Source :
-
Cancer Genetics & Cytogenetics . Oct2008, Vol. 186 Issue 2, p78-84. 7p. - Publication Year :
- 2008
-
Abstract
- Abstract: Genistein is a major soy isoflavone with multiple properties. The impact of soy genistein on breast cancer is controversial. One of the issues is whether soy genistein has a genotoxic effect at physiological concentrations. To address this question using an in vitro model, we first established MCF-10A/G0 and MCF-10A/G1 cell lines, which were MCF-10A cells exposed to 0.01% dimethyl sulfoxide (as vehicle control), i.e., MCF-10A/G0, or 1 μmol/L of genistein for 3 months, MCF-10A/G1, respectively. Chromosomal changes were compared between the two cell lines by routine G-banded chromosome analyses and both regular and array-based comparative genomic hybridization. After 3 months of exposure to genistein, the cell line MCF-10A/G1 showed loss of a normal chromosome 8 and gain of an extra chromosome 20, as well as loss of a chromosomal segment on the short arm of chromosome 9, leading to a homozygous deletion of the tumor suppressor genes CDKN2A (alias p16INK4a ) and CDKN2B (alias p15INK4b ). Our results suggest that long-term, low-concentration exposure to genistein may have the potential to induce chromosomal imbalances. These genotoxic effects may work in concert with other factors to induce genetic lesions that contribute to soy- and genistein-associated risk. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01654608
- Volume :
- 186
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Cancer Genetics & Cytogenetics
- Publication Type :
- Academic Journal
- Accession number :
- 34742675
- Full Text :
- https://doi.org/10.1016/j.cancergencyto.2008.06.010