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Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system.
- Source :
-
Journal of Industrial Microbiology & Biotechnology . Nov2008, Vol. 35 Issue 11, p1345-1352. 8p. 1 Black and White Photograph, 3 Charts, 2 Graphs. - Publication Year :
- 2008
-
Abstract
- The applicability of order-specific primers in minimizing the possible underestimation of microbial diversity was evaluated via denaturing gradient gel electrophoresis (DGGE) analysis of a lab-scale anaerobic digester. Initially, a population analysis with real-time quantitative PCR demonstrated the existence of three methanogenic orders— Methanobacteriales, Methanomicrobiales, and Methanosarcinales—throughout the reaction period. DGGE analyses with three pairs of order-specific primers yielded eight operational taxonomic units (OTUs), whereas DGGE analysis with two independent Archaea-specific primers identified only five. Moreover, the order-specific primers amplified at least one OTU affiliated with each order, whereas no members of Methanobacteriales or Methanomicrobiales were identified with Archaea-specific primers in most samples. These findings provide evidence that order-specific analysis can detect the diversity of methanogens in greater detail than conventional Archaea-specific analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 35
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 34729269
- Full Text :
- https://doi.org/10.1007/s10295-008-0417-7