Impact of seasonal variation on HSP70 expression quantitated in stressed fish hepatocytes

Abstract: Heat shock protein 70 (HSP70) is an effective molecular chaperone, playing a role in cell protection from damage in response to stress stimuli. Here, we report the impact of environmental stress on hepatocyte HSP70 expression in Mugil cephalus living in either a contaminated (Ennore) or uncontaminated (Kovalam) estuary over the course of two seasons. Oxidative and nitrative stress was determined along with quantification of HSP70 by enzyme-linked immunosorbent assay (ELISA) after electroelution from polyacrylamide gels. Fish from Ennore showed significantly higher levels of oxidative and nitrative stress and HSP70 expression than fish from Kovalam. Also, there was significant seasonal variation in all oxidative, nitrative stress marker levels and HSP70 expression which peaked during summer. These results provide direct evidence that HSP70 overexpression in fish hepatocytes under stress may aid cell survival by protecting against oxidative and nitrative stress-induced changes. In addition, seasonal variation may have a significant impact on HSP70 expression. [Copyright &y& Elsevier]