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Binding Site Analysis of Cellulose Binding Domain CBDN1 from Endoglucanase C of Cellulomonas fimi...
- Source :
-
Biochemistry . 08/01/2000, Vol. 39 Issue 30, p8844. 9p. 2 Black and White Photographs, 2 Diagrams, 2 Charts, 7 Graphs. - Publication Year :
- 2000
-
Abstract
- Evaluates the contributions of 10 amino acids within the binding cleft of one of the cellulose binding domains of the soil bacterium Cellulomonas fimi. Single site-directed mutations to alanine residues; Similar mechanism of binding to soluble and insoluble substrates; Importance of tyrosines 19 and 85 for tight binding.
- Subjects :
- *AMINO acids
*CELLULOSE
*CELLULOMONAS
*SITE-specific mutagenesis
*ALANINE
Subjects
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 39
- Issue :
- 30
- Database :
- Academic Search Index
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 3425502
- Full Text :
- https://doi.org/10.1021/bi000607s