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Aliphatic β-nitro alcohols for non-enzymatic collagen cross-linking of scleral tissue
- Source :
-
Experimental Eye Research . Sep2008, Vol. 87 Issue 3, p279-285. 7p. - Publication Year :
- 2008
-
Abstract
- Abstract: The success of riboflavin photochemical cross-linking of the cornea in treating keratoconus and post-surgical keratectasia has prompted interest in cross-linking scleral tissue with a potential application to stabilize myopic progression. Applying an UVA light source to the sclera is difficult, particularly in the posterior region. An alternate pharmacologic approach to scleral cross-linking may be possible. The present study was undertaken in order to identify nitrite related compounds capable of inducing scleral tissue cross-linking and to gain information regarding the possible chemical mechanisms involved. 8×4mm strips of porcine and human sclera were incubated in various concentrations of nitrite related agents (1–100mM) at 37°C. pH 7.4 was used for all experiments except those involving NaNO2. Following a 24–96h incubation period, the samples were tested for cross-linking effects using thermal shrinkage temperature (T s) analysis. Several compounds were studied including NaNO2, 2-nitroethanol, 2-nitro-1-propanol, 3-nitro-2-pentanol, 2-nitrophenol, 2-nitroethane, 2-aminoethanol, isopentyl nitrite, DPTA/NO, DETA/NO, and urea, a nitrous acid trap. The results indicate that short chain aliphatic β-nitro alcohols (2-nitroethanol, 2-nitro-1-propanol, and 3-nitro-2-pentanol) are particularly effective cross-linking agents at pH 7.4, showing both time and concentration dependent effects. Furthermore, nitrosation does not appear to induce tissue cross-linking. In conclusion, aliphatic β-nitro alcohols can cross-link scleral tissue at physiologic pH and temperature. Since β-nitro alcohols are known to have reasonable toxicity profiles, these agents could find utility as pharmacologic cross-linking agents for scleral thinning disease. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00144835
- Volume :
- 87
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Experimental Eye Research
- Publication Type :
- Academic Journal
- Accession number :
- 34211183
- Full Text :
- https://doi.org/10.1016/j.exer.2008.06.008