Back to Search
Start Over
Genetic diversity of the VP1 gene of duck hepatitis virus type I (DHV-I) isolates from southeast China is related to isolate attenuation
- Source :
-
Virus Research . Oct2008, Vol. 137 Issue 1, p137-141. 5p. - Publication Year :
- 2008
-
Abstract
- Abstract: The complete sequence of an isolate (ZJ-V) of Duck hepatitis virus I (DHV-I), originally taken from the field in southeast China was determined. It was 7691 nucleotides long and had 5′- and 3′-terminal non-coding regions of 626 and 315 nucleotides, respectively. The poly(A) tail contained at least 22 residues and the single open reading frame encoded a polypeptide of 2249 amino acids. The VP1 gene was also sequenced from nine southeast China field isolates and three attenuated DHV-I vaccine strains. In phylogenetic analysis of the isolates and other published sequences, attenuated and tissue-adapted isolates (including ZJ-V) clustered as genotypes significantly different from the field isolates that had not been passaged in chicken/duck embryos. There were two consistent amino acid substitutions (E129 →V129 and A142 →S142) between all the field isolates and all the tissue-adapted ones. The carboxyl terminal region was generally the most variable and here the four attenuated Chinese isolates showed six consistent differences from the field isolates (S181 →L181, H183K184 →R183G1841, N193 →D193, E205 →K205, R217 →K217, N235 →D235). It seems likely that at least some of these differences result from mutations leading to isolate attenuation. [Copyright &y& Elsevier]
- Subjects :
- *NUCLEIC acids
*GENETIC polymorphisms
*HEPATITIS viruses
*LIVER diseases
Subjects
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 137
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 34085159
- Full Text :
- https://doi.org/10.1016/j.virusres.2008.04.030