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Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells.
- Source :
-
Kidney International . Sep2008, Vol. 74 Issue 6, p808-816. 9p. 2 Color Photographs, 5 Graphs. - Publication Year :
- 2008
-
Abstract
- We previously showed that a local immune response largely composed of type 1 T cells correlated with a favorable outcome of the peritonitis associated with peritoneal dialysis. To clarify how these subsets are recruited to the peritoneal cavity during inflammation, we measured integrin-mediated interactions between the T cells and human peritoneal mesothelial cells. Direct microscopy showed that lipopolysaccharide or peritoneal dialysis effluent stimulated the adherence of T cells to mesothelial cells, a process mediated by the integrins α6β1 and α4β1. Further, the migration of Th1 cell across human mesothelial cell monolayers grown on transwell surfaces was reduced by anti-α6β1 integrin antibody while that of Th2 cell was inhibited by an anti-α4 integrin antibody. Pretreatment with either lipopolysaccharide or rapid response peritoneal dialysis effluent stimulated T cell migration and this was significantly decreased by the α6β1 compared to the α4 antibody. These results suggest that integrins may play an important role in mediating selective T cell subset adhesion and migration across human peritoneal mesothelial cell monolayers and differential integrin expression and selective T cell subset recruitment during peritonitis may affect outcome.Kidney International (2008) 74, 808–816; doi:10.1038/ki.2008.330; published online 9 July 2008 [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00852538
- Volume :
- 74
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Kidney International
- Publication Type :
- Academic Journal
- Accession number :
- 34051623
- Full Text :
- https://doi.org/10.1038/ki.2008.330