HPV DNA TYPING STUDY IN ASCUS: SOME CONSIDERATIONS ON AGE GROUPS. (PRELIMINARY NOTES BY THE PROGRAM OF EXCELLENCE IN RESEARCH OF ROMANIAN MINISTRY OF EDUCATION AND RESEARCH).

Objectives: To individualize a follow-up methodology for ASCUS Pap-tests in relationship with the viral type and age groups of patients. Materials and Methods: HPV DNA typing by PCR for women with the cervical lesions, Pap-test: conventional, LBC in last six months. From 175 cases with HPV DNA typing, 41 have been confirmed as ASCUS and 4 cases as ASC-H. We have divided patients in 3 age groups: between 20-30 years old (13 cases), between 31-40 years old (22 cases), over 40 years old (10 cases). Results: HPV DNA typing (PCR) 20 negative results, one type of HPV 15 cases, multiple viral types 10 cases: 2 types: 3 women; 3 types: 6 women; 5 types: 1 women: ASC-H4 cases: 2 cases: with HPV-16, 2 with multiple types all within the 31-40 years old segment; ASCUS-21 cases: 13 with one type, 8 cases with more viral types (including HR HPV in 5 cases), HPV DNA typing (PCR) - 25 positive results. Conclusions: The age group of 31-40 years old offers high number of ASCUS with positive HPV DNA type. No ASC-H with HPV negative. The colposcopy result helps and indicates the biopsy in the false negative HPV DNA typing. The age group of 31-40 years olds with ASCUS persistent results and HPV negative has to be followed more than 24 months and a safe attitude could be the treatment by LLETZ or conization. It is known that in postmenopausal period HPV DNA is useful and could help if cytology or colposcopy are unsatisfactory. [ABSTRACT FROM AUTHOR]