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Roles of Wnt/β-catenin signaling in adipogenic differentiation potential of adipose-derived mesenchymal stem cells
- Source :
-
Molecular & Cellular Endocrinology . Sep2008, Vol. 291 Issue 1/2, p116-124. 9p. - Publication Year :
- 2008
-
Abstract
- Abstract: Wnt/β-catenin signaling pathway controls differentiation of various cells by regulating the expression of target genes. β-Catenin plays a central role in Wnt/β-catenin signaling pathway. To investigate the molecular mechanisms of fate determination in adipose-derived mesenchymal stem cells (AMSCs), we investigated effects of Wnt3a and β-catenin, two key members of the Wnt/β-catenin signaling, in adipogenic differentiation of porcine AMSCs. We demonstrated that Wnt3a protein can inhibit the adipogenic differentiation of porcine AMSCs in vitro culture. By stabilization of cytoplasmic β-catenin with continuous treatment by LiCl, the adipogenic differentiation of AMSCs was also suppressed and the osteogenesis was stimulated. In contrast, a loss of β-catenin in AMSCs enhanced the adipogenic differentiation and rescued LiCl-induced anti-adipogenesis. In addition, the mutual activation of CCAAT/enhancer-binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor-γ (PPARγ) were repressed in the presence of Wnt3a or LiCl, but increased in the gene silencing of β-catenin. Taken together, our study indicated that Wnt/β-catenin signaling pathway inhibited the adipogenic differentiation potential and alter the cell fate from adipocytes to osteoblasts. [Copyright &y& Elsevier]
- Subjects :
- *STEM cells
*FAT cells
*GENETIC regulation
*PORCINE somatotropin
Subjects
Details
- Language :
- English
- ISSN :
- 03037207
- Volume :
- 291
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Molecular & Cellular Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 33530231
- Full Text :
- https://doi.org/10.1016/j.mce.2008.05.005