Cell-Based Fluorescence Screen for K+ Channels and Transporters Using an Extracellular Triazacryptand-Based K+ Sensor.

The article reports on cell-based fluorescence screen for K+ channels and transporters using an extracellular triazacryptand-based K+ sensor. The authors report the synthesis of a water-soluble, dextran-conjugated fluorescent K+ sensor, TAC-Limedex, whose green fluorescence strongly increases with K+ and demonstrate its utility for assay of cellular K+.