A cryptic microbial community persists within micropropagated Bouteloua eriopoda (Torr.) Torr. cultures

Abstract: Higher plants are ubiquitously colonized with fungal endophytes that often lack readily detectable structures. This study examines the diverse endophyte population within a single line of micropropagated Bouteloua eriopoda (Torr.) Torr., using microscopy and comparison of internal spacer (ITS) gene sequences obtained from both plant and isolated fungal tissues. Microscopy revealed fungal hyphae and lipid bodies, the majority of which lacked distinguishing characters. Internal transcribed spacer (ITS) sequences amplified from fungal isolates and micropropagated plant tissues were subjected to Bayesian analysis, which clearly distinguished six endophyte taxa. Results confirm a diverse, cryptic endophyte consortium is retained within this micropropagated plant line. The probability of similar complexity in other plant species is discussed. The development of controlled systems in which to study single plant-fungal interactions within such consortia presents significant technical challenges. However, potential for such systems to reveal species interactions that influence plant growth and development is high. [Copyright &y& Elsevier]