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Immunoproteomic assay of surface proteins of Streptococcus suis serotype 9.
- Source :
-
FEMS Immunology & Medical Microbiology . Jun2008, Vol. 53 Issue 1, p52-59. 8p. 2 Black and White Photographs, 4 Charts, 1 Graph. - Publication Year :
- 2008
-
Abstract
- Streptococcus suis is an important swine pathogen responsible for a diverse group of diseases. Studies on vaccines have focused on S. suis serotype 2 strains, which are the most invasive isolates worldwide. However, in China S. suis serotype 9 (SS9) is also a prevalent serotype, which is frequently isolated from diseased pigs. Little is known about immunogenic proteins for SS9. Therefore, an immunoproteomic-based approach was developed to identify immunogenic proteins of SS9. Cell wall proteins extracted from SS9 strain GZ0565 isolated from a diseased pig with meningitis were screened by two-dimensional Western blotting using anti-SS9 sera pooled from specific pathogen-free mice. Protein spots were excised from preparative gels and identified by matrix-assisted laser desorption ionization time-of-flight MS (MALDI-TOF-MS) or MALDI-TOF-TOF-MS, which led to the identification of eight immunogenic proteins (arginine deiminase, extracellular solute-binding protein, translation elongation factor Ts, neprilysin, peptide ATP-binding cassette transporter peptide-binding protein, pyruvate kinase, phosphate acetyltransferase, and fructose-bisphosphate aldolase). These immunogenic proteins, which are encoded by genes that are reasonably conserved among SS9 strains, could be developed as vaccine candidates. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09288244
- Volume :
- 53
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- FEMS Immunology & Medical Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32000441
- Full Text :
- https://doi.org/10.1111/j.1574-695X.2008.00401.x