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Glutamate Uptake and Release by Astrocytes Are Enhanced by Clostridium botulinum C3 Protein.
- Source :
-
Journal of Biological Chemistry . 4/4/2008, Vol. 283 Issue 14, p9289-9299. 11p. 2 Diagrams, 4 Graphs. - Publication Year :
- 2008
-
Abstract
- Inhibition of Rho activity by Clostridium botulinum C3 transferase (C3bot) versatily changes functional properties of neural cells. Using cultivated mouse astrocytes, we show here that C3bot increases both uptake and secretion of glutamate. The enhanced glutamate uptake is initiated by an NFκB-dependent up-regulation of the glial glutamate transporter 1 that is efficaciously sorted to the plasma membrane. The increase in cytosolic glutamate concentration promotes vesicular glutamate storage in astrocytes treated with C3bot. Parallel to the increased storage, C3-induced impairment of Rho-dependent pathways strongly enhances Ca2+-dependent secretion of glutamate. This is accompanied by higher levels of the SNARE protein synaptobrevin. Synaptobrevin inactivation by botulinum neurotoxin D almost completely inhibits Ca2+-dependent glutamate secretion triggered by C3bot, indicating that the enhanced release of glutamate mainly originates from exocytosis. In addition, C3bot increases the exocytosis/endocytosis turnover, as analyzed by the stimulated accumulation of the fluorescent dye AM1-43. The release of glutamine, the main metabolite of glutamate, is only moderately affected by C3bot. In conclusion, inhibition of Rho-dependent pathways shifts astrocytes to a secretory active stage in which they may modulate neuronal excitability. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CLOSTRIDIUM
*CELLS
*BOTULINUM toxin
*NEURAL stem cells
*EXCRETION
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 283
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31963999
- Full Text :
- https://doi.org/10.1074/jbc.M706499200