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An Enantiomer-Selective Liquid Chromatography—Tandem Mass Spectrometry Method for Methadone and EDDP Validated for Use in Human Plasma, Urine, and Liver Microsomes.
- Source :
-
Journal of Analytical Toxicology . Apr2008, Vol. 32 Issue 3, p208-219. 12p. 7 Charts, 5 Graphs. - Publication Year :
- 2008
-
Abstract
- The article discusses the development and validation of a liquid chromatography-electrospray ionization-tandem mass spectrometry method to detect (R)- and (S)-methadone and (R)- and (S)-2ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in human plasma with cross substantiation to urine and liver microsomes. It is suggested that the use of deuterated internal standards and liquid-liquid extraction matched with chiral separation allowed for baseline separation with a lower limit of quantitation (LLOQ) of 2.5ng/ml.
Details
- Language :
- English
- ISSN :
- 01464760
- Volume :
- 32
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Analytical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 31877133
- Full Text :
- https://doi.org/10.1093/jat/32.3.208