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A Controllable Solid-State Ru(bpy)32Electrochemiluminescence Film Based on Conformation Change of Ferrocene-Labeled DNA Molecular Beacon.
- Source :
-
Langmuir . Mar2008, Vol. 24 Issue 5, p2200-2205. 6p. - Publication Year :
- 2008
-
Abstract
- A controllable solid-state electrochemiluminescence (ECL) film based on efficient and stable quenching of ECL of ruthenium(II) tris-(bipyridine) (Ru(bpy)32) by oxidizing ferrocene (Fc) at the electrode is developed. The ECL intensity is correlated to the distance which is controlled by the conformation of the ferrocene-labeled DNA molecular beacon (Fc-MB) between the Fc and Ru(bpy)32immobilized on the electrode. The conformation adjustment is conducted viacomplementary DNA hybridizing with the bases in the loop of the Fc-MB and changing the temperature of the Fc-MB and the resultant double-stranded DNA (dsDNA). Those events all result in change of the ECL intensity. With such characteristics, the solid-state Ru(bpy)32-ECL film has the potential to be applied to reagentless DNA ECL biosensors and to calculate thermodynamic parameters of equilibrium constants of MB binding and the stem-loop formation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NUCLEIC acids
*BIOMOLECULES
*GENETIC transformation
*NUCLEOTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 07437463
- Volume :
- 24
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Langmuir
- Publication Type :
- Academic Journal
- Accession number :
- 31844630
- Full Text :
- https://doi.org/10.1021/la702345p