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A sensitive amperometric immunosensor for carcinoembryonic antigen detection with porous nanogold film and nano-Au/chitosan composite as immobilization matrix
- Source :
-
Journal of Proteomics . Apr2008, Vol. 70 Issue 6, p823-829. 7p. - Publication Year :
- 2008
-
Abstract
- Abstract: A sensitive amperometric immunosensor for carcinoembryonic antigen (CEA) was prepared. Firstly, a porous nano-structure gold (NG) film was formed on glassy carbon electrode (GCE) by electrochemical reduction of HAuCl4 solution, then nano-Au/Chit composite was immobilized onto the electrode because of its excellent membrane-forming ability, and finally the anti-CEA was adsorbed onto the surface of the bilayer gold nanoparticles to construct an anti-CEA/nano-Au/Chit/NG/GCE immunosensor. The characteristics of the modified electrode at different stages of modification were studied by cyclic voltammetry (CV). The gold colloid, chitosan and nano-Au/Chit were characterized by transmission electron microscopy and UV–vis spectroscopy. In addition, the performances of the immunosensor were studied in detail. The resulting immunosensor offers a high-sensitivity (1310 nA/ng/ml) for the detection of CEA and has good correlation for detection of CEA in the range of 0.2 to 120.0 ng/ml with a detection limit of 0.06 ng/ml estimated at a signal-to-noise ratio of 3. The proposed method can detect the CEA through one-step immunoassay and would be valuable for clinical immunoassay. [Copyright &y& Elsevier]
- Subjects :
- *IMMUNOASSAY
*IMMUNOGLOBULINS
*ELECTRON microscopy
*NANOPARTICLES
Subjects
Details
- Language :
- English
- ISSN :
- 18743919
- Volume :
- 70
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 31750900
- Full Text :
- https://doi.org/10.1016/j.jbbm.2007.06.002