Thermodynamic Analysis of Cavity Creating Mutations in an Engineered Leucine Zipper and...

Reports on the energetic consequences of a cavity introduced by Leu/Ala substitution at the tightly packed dimeric interface and how addition of 30% glycerol affects the folding thermodynamics of the engineered dimeric leucine zipper A2 and the cavity mutants. Indication that mutational and solvent effects on protein stability can be thermodynamically complex.