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Increased concentrations of the oxidative DNA adduct 7,8-dihydro-8-oxo-2-deoxyguanosine in the germ-line of men with type 1 diabetes.
- Source :
-
Reproductive BioMedicine Online (Reproductive Healthcare Limited) . Mar2008, Vol. 16 Issue 3, p401-409. 9p. - Publication Year :
- 2008
-
Abstract
- The effects of diabetes mellitus on male reproductive health have not been clearly defined. A previous publication from this group reported significantly higher levels of nuclear DNA fragmentation and mitochoodrial DNA deletions in spermatozoa from men with type 1 diabetes. This study compared semen profiles, sperm DNA fragmentation and levels of oxidative DNA modification in spermatozoa of diabetic and non-diabetic men. Semen samples from 12 non-diabetic, fertile men and 11 type 1 diabetics were obtained and subjected to conventional light microscopic semen analysis. Nuclear DNA fragmentation was assessed using an alkaline Comet assay and concentrations of 7,8-dihydro-8-oxo-2-deoxyguanosine (8-OHdG), an oxidative adduct of the purine guanosine, were assessed by high-performance liquid chromatography. Conventional semen profiles were similar in both groups, whilst spermatozoa from type 1 diabetics showed significantly higher levels of DNA fragmentation (44% versus 27%; P < 0.05) and concentrations of 8-OHdG (3.6 versus 2.0 molecules of 8-OHdG per 105 molecules of deoxyguanosine; P < 0.05). Furthermore, a positive correlation was observed between DNA fragmentation and concentrations of 8-OHdG per 105 molecules of deoxyguanosine (rs = 0.7, P < 0.05). The genomic damage evident in spermatozoa of type 1 diabetics may have important implications for their fertility and the outcome of pregnancies fathered by these individuals. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA adducts
*DIABETES
*SPERMATOZOA
*DNA
*OXIDATIVE stress
Subjects
Details
- Language :
- English
- ISSN :
- 14726483
- Volume :
- 16
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Reproductive BioMedicine Online (Reproductive Healthcare Limited)
- Publication Type :
- Academic Journal
- Accession number :
- 31466116
- Full Text :
- https://doi.org/10.1016/S1472-6483(10)60602-5