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Naringenin Protects HaCaT Human Keratinocytes Against UVB-induced Apoptosis and Enhances the Removal of Cyclobutane Pyrimidine Dimers from the Genome.
- Source :
-
Photochemistry & Photobiology . Mar2008, Vol. 84 Issue 2, p307-316. 10p. 1 Black and White Photograph, 1 Diagram, 5 Graphs. - Publication Year :
- 2008
-
Abstract
- Many naturally occurring agents are believed to protect against UV-induced skin damage. In this study, we have investigated the effects of naringenin (NG), a naturally occurring citrus flavonone, on the removal of UVB-induced cyclobutane pyrimidine dimers (CPD) from the genome and apoptosis in immortalized p53-mutant human keratinocyte HaCaT cells. The colony-forming assay shows that treatment with NG significantly increases long-term cell survival after UVB irradiation. NG treatment also protects the cells from UVB-induced apoptosis, as indicated by the absence of the 180 base pair DNA ladders and the appearance of sub-G1 peak using agarose gel electrophoresis and flow cytometric analysis, respectively. The UVB-induced poly (ADP-ribose) polymerase-1 (PARP-1) cleavage, caspase activation and Bax/Bcl2 ratio were modulated following NG treatment, indicating an antiapoptotic effect of NG in UVB-damaged cells that occurs at least in part via caspase cascade pathway. Moreover, treatment of UVB-irradiated HaCaT cells with NG enhances the removal of CPD from the genome, as observed by both direct quantitation of CPD in genomic DNA and immuno-localization of the damage within the nuclei. The study provides a molecular basis for the action of NG as a promising natural flavonoid in preventing skin aging and carcinogenesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KERATINOCYTES
*GENOMES
*GENES
*ULTRAVIOLET radiation
*GENETICS
Subjects
Details
- Language :
- English
- ISSN :
- 00318655
- Volume :
- 84
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Photochemistry & Photobiology
- Publication Type :
- Academic Journal
- Accession number :
- 31308789
- Full Text :
- https://doi.org/10.1111/j.1751-1097.2007.00255.x