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Probing the dynamic fluorescence properties of single water-soluble quantum dots
- Source :
-
Optics Communications . Apr2008, Vol. 281 Issue 7, p1781-1788. 8p. - Publication Year :
- 2008
-
Abstract
- Abstract: The influence of surface modifications, including carboxyl-, amine-, and mercaptoundecanoicacid-functionalized chemistries, on the dynamic fluorescence properties of single quantum dots (QDs) are probed using a combined confocal fluorescence microscopy and spectroscopy system. Water-soluble QDs with carboxyl- or amine-functionalized surfaces prepared through a linker arm show significant fluorescence intermittency and appear statistically identical. In contrast, water-soluble QDs functionalized via mercaptoundecanoicacid, an electron-donating thiol group, demonstrate suppressed fluorescence intermittency suggesting the photostability of water-soluble QDs is strongly dependant upon the method of surface functionalization. In addition, the long-term photostability of the mercaptoundecanoicacid-functionalized QD demonstrates an excitation-time-dependent blue-shift in the fluorescence emission spectra. Our measurement and analysis techniques promise a wealth of insight in elucidating the photophysics of bio-conjugated QDs and assessing and designing bio-conjugated QDs for biomedical applications requiring long-term photostabililty. [Copyright &y& Elsevier]
- Subjects :
- *FLUORESCENCE
*QUANTUM dots
*ELECTRONS
*FLUORESCENCE microscopy
Subjects
Details
- Language :
- English
- ISSN :
- 00304018
- Volume :
- 281
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Optics Communications
- Publication Type :
- Academic Journal
- Accession number :
- 30907948
- Full Text :
- https://doi.org/10.1016/j.optcom.2007.07.068