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Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells.

Authors :
ShangJie Wu
Shu Duan
ShuiPing Zhao
Ying Cai
Ping Chen
Xiang Fang
Source :
Respiratory Research. 2005, Vol. 6, p27-6. 6p.
Publication Year :
2005

Abstract

Objective: To explore the effects of atorvastatin on expression of cyclooxygenase-2 (COX-2) in human pulmonary epithelial cells (A549). Methods: A549 cells were incubated in DMEM medium containing lipopolysaccharide (LPS) in the presence or absence of atorvastatin. After incubation, the medium was collected and the amount of prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assay (ELISA). The cells were harvested, and COX-2 mRNA and protein were analyzed by RT-PCR and western-blot respectively. Results: LPS increased the expression of COX-2 mRNA and production of PGE2 in a dose- and time-dependent manner in A549. Induction of COX-2 mRNA and protein by LPS were inhibited by atorvastatin in a dose-dependent manner. Atorvastatin also significantly decreased LPS-induced production of PGE2. There was a positive correlation between reduced of COX-2 mRNA and decreased of PGE2 (r = 0.947, P < 0.05). Conclusion: Atorvastatin down-regulates LPS-induced expression of the COX-2 and consequently inhibits production of PGE2 in cultured A549 cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14659921
Volume :
6
Database :
Academic Search Index
Journal :
Respiratory Research
Publication Type :
Academic Journal
Accession number :
30735719
Full Text :
https://doi.org/10.1186/1465-9921-6-27