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Calcium Phosphate—DNA Nanocomposites: Morphological Studies and Their Bile Duct Infusion for Liver-Directed Gene Therapy.

Authors :
Singh, Ruchi
Saxena, Amit
Mozumdar, Subho
Source :
International Journal of Applied Ceramic Technology. Jan2008, Vol. 5 Issue 1, p1-10. 10p. 1 Color Photograph, 1 Black and White Photograph, 1 Diagram, 4 Graphs.
Publication Year :
2008

Abstract

In this study, liver-directed gene transfer in rats with calcium phosphate (CaP) nanoparticles and the effect of the route of administration and surgical manipulations on transfection efficiency is reported. Formulations of CaP nanoparticles entrapping plasmid DNA (pDNA) were prepared by the reverse micellar method using two different surfactants. Transmission electron microscopy, scanning electron microscopy and dynamic light scattering were used to characterize the CaP–DNA nanocomposites. The morphological characteristics of the formulations showed a strong dependency on temperature. Gel electrophoresis experiments indicated that there was no degradation of the encapsulated pDNA, and in vitro cell transfection in HEK-293 and primary hepatocytes from rats as well as in vivo intraductal delivery experiments suggested that CaP nanoparticles led to significant and prolonged transgene expression. Therefore, our methodology gives a stable and viable formulation for hepatic gene therapy. Low-DNA dosage entrapped in CaP nanoparticles makes it an effective gene delivery system for clinical applications. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1546542X
Volume :
5
Issue :
1
Database :
Academic Search Index
Journal :
International Journal of Applied Ceramic Technology
Publication Type :
Academic Journal
Accession number :
29993609
Full Text :
https://doi.org/10.1111/j.1744-7402.2008.02183.x