Convenient Transformation of Anamorphic Basidiomycetous Yeasts Belonging to Genus Pseudozyma Induced by Electroporation

A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/μg of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure. [Copyright &y& Elsevier]