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Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles
- Source :
-
Virology . Feb2008, Vol. 371 Issue 1, p108-120. 13p. - Publication Year :
- 2008
-
Abstract
- Abstract: Sendai virus (SeV) is unique in that one of the viral accessory proteins, C, enhances budding of virus-like particles (VLPs) formed by SeV matrix protein M by physically interacting with Alix/AIP1. C protein itself does not have the ability to form VLPs, while M protein provides viral budding force, like other enveloped viruses. Here we show that SeV C protein recruits Alix/AIP1 to the plasma membrane (PM) to facilitate VLP budding. SeV M-VLP budding is sensitive to overexpression of a dominant-negative (DN) form of VPS4A only in the presence of the C proteins, which is able to recruit Alix/AIP1 to the PM. Our results indicate that SeV M and C proteins play separate roles in the budding process: M protein drives budding and C protein enhances the efficiency of the utilization of cellular MVB sorting machinery for efficient VLP budding. [Copyright &y& Elsevier]
- Subjects :
- *SENDAI virus
*CELL membranes
*EXTRACELLULAR matrix proteins
*VIRUSES
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 371
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 28397618
- Full Text :
- https://doi.org/10.1016/j.virol.2007.09.020