A Luminescent Sensor for Tyrosine Phosphorylation.

We have developed a luminogenic probe for tyrosine phosphorylation based on a short peptide sequence containing an iminodiacetate moiety near the site of phosphorylation. In response to kinase activity, the probe provides a strong luminescence enhancement, resulting from the increased ability of the probe to bind and sensitize Tb3and Eu3ions upon phosphorylation. [ABSTRACT FROM AUTHOR]