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Canonical insertion-deletion markers for rapid DNA typing of Francisella tularensis.
- Source :
-
Emerging Infectious Diseases . Nov2007, Vol. 13 Issue 11, p1725-1732. 8p. 3 Diagrams, 1 Chart, 1 Graph. - Publication Year :
- 2007
-
Abstract
- To develop effective and accurate typing of strains of Francisella tularensis, a potent human pathogen and a putative bioterrorist agent, we combined analysis of insertion-deletion (indel) markers with multiple-locus variable-number tandem repeat analysis (MLVA). From 5 representative F. tularensis genome sequences, 38 indel markers with canonical properties, i.e., capable of sorting strains into major genetic groups, were selected. To avoid markers with a propensity for homoplasy, we used only those indels with 2 allelic variants and devoid of substantial sequence repeats. MLVA included sequences with much diversity in copy number of tandem repeats. The combined procedure allowed subspecies division, delineation of clades A.I and A.II of subspecies tularensis, differentiation of Japanese strains from other strains of subspecies holarctica, and high-resolution strain typing. The procedure uses limited amounts of killed bacterial preparations and, because only 1 single analytic method is needed, is time- and cost-effective. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FRANCISELLA tularensis
*PATHOGENIC microorganisms
*BIOTERRORISM
*GENOMES
*NUCLEOTIDE sequence
*GENETICS
*RESEARCH
*GENETIC mutation
*BIOLOGICAL evolution
*GRAM-negative bacteria
*DNA fingerprinting
*RESEARCH methodology
*MEDICAL cooperation
*EVALUATION research
*DNA probes
*COMPARATIVE studies
*POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 10806040
- Volume :
- 13
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Emerging Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 27495657
- Full Text :
- https://doi.org/10.3201/eid1311.070603