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Detection and validation of EST-derived SNPs for poplar leaf rust Melampsora medusae f. sp. deltoidae.

Authors :
Feau, Nicolas
Bergeron, Marie-Josée
Joly, David L.
Roussel, Fabien
Hamelin, Richard C.
Source :
Molecular Ecology Notes. Dec2007, Vol. 7 Issue 6, p1222-1228. 7p. 2 Charts.
Publication Year :
2007

Abstract

We report the discovery, characterization and validation of 118 single nucleotide polymorphisms (SNPs) for poplar leaf rust Melampsora medusae f. sp. deltoidae identified using a gene-targeted approach in an expressed sequence tag (EST) library. We developed a genotyping assay using the iPLEX™ primer extension method for two multiplex assays of 28 and 22 SNPs. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
*NUCLEOTIDES
*MELAMPSORA
*MELAMPSORACEAE
*RUST fungi
*GENETIC polymorphisms
*GENETICS

Details

Language :
English
ISSN :
14718278
Volume :
7
Issue :
6
Database :
Academic Search Index
Journal :
Molecular Ecology Notes
Publication Type :
Academic Journal
Accession number :
27335948
Full Text :
https://doi.org/10.1111/j.1471-8286.2007.01838.x
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