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Differential protein expression in ovaries of uninfected and Babesia-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus
- Source :
-
Insect Biochemistry & Molecular Biology . Dec2007, Vol. 37 Issue 12, p1291-1308. 18p. - Publication Year :
- 2007
-
Abstract
- Abstract: We used gel electrophoresis and mass spectrometry to investigate differences in protein expression in ovarian tissues from Babesia bovis-infected and uninfected southern cattle tick, Rhipicephalus (Boophilus) microplus. Soluble and membrane proteins were extracted from ovaries of adult female ticks, and analyzed by isoelectric focusing (IEF) and one-dimensional or two-dimensional (2-D) gel electrophoresis. Protein patterns were analyzed for differences in expression between infected and uninfected ticks. 2-D separation of proteins revealed a number of proteins that appeared to be up- or down-regulated in response to infection with Babesia, in particular membrane/membrane-associated proteins and proteins in a low molecular mass range between 6 and 36kDa. A selection of differentially expressed proteins was subjected to analysis by capillary-HPLC-electrospray tandem mass spectrometry (HPLC-ESI-MS/MS). Among the ovarian proteins that were up-regulated in infected ticks were calreticulin, two myosin subunits, an endoplasmic reticulum protein, a peptidyl-prolyl cis–trans isomerase (PPIase), a cytochrome c oxidase subunit, a glutamine synthetase, and a family of Kunitz-type serine protease inhibitors. Among the down-regulated ovarian proteins were another PPIase, a hemoglobin subunit, and a lysozyme. This study is part of an ongoing effort to establish a proteome database that can be utilized to investigate specific proteins involved in successful pathogen transmission. [Copyright &y& Elsevier]
- Subjects :
- *BIOMOLECULES
*ELECTROPHORESIS
*AMINO acids
*MASS (Physics)
Subjects
Details
- Language :
- English
- ISSN :
- 09651748
- Volume :
- 37
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Insect Biochemistry & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 27242088
- Full Text :
- https://doi.org/10.1016/j.ibmb.2007.08.001