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Verticase: a Fibrinolytic Enzyme Produced by Verticillium sp. Tj33, an Endophyte of Trachelospermum jasminoides.
- Source :
-
Journal of Integrative Plant Biology . Nov2007, Vol. 49 Issue 11, p1548-1554. 7p. 2 Black and White Photographs, 2 Charts, 4 Graphs. - Publication Year :
- 2007
-
Abstract
- Plant endophytes are among the most important resources of biologically active metabolites. Twenty-three endophyte strains residing in Trachelospermum jasminoides were cultivated in vitro with the cultures assayed for the fibrinolytic substance production. As a result, the culture of Verticillium sp. Tj33 was shown to be the most active. A fibrinolytic enzyme designated as verticase was subsequently purified from the supernatant of Verticillium sp. culture broth by a combination of DEAE-52, Sephadex G-75 and hydrophobic column chromatographies. Verticase, with its molecular mass of 31 kDa and pI of 8.5, was demonstrated to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing electrophoresis. Verticase is an enzyme that hydrolyzes fibrin directly without activation of plaminogen. It was stable in a broad pH range from 4 through to 11 with the optimal reaction pH value and temperature shown to be around 9–10 and 50–60 °C, respectively. The fibrinolytic activity of verticase was severely inhibited by phenylmethylsulfony fluoride, indicating that verticase was a serine protease. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16729072
- Volume :
- 49
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Integrative Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 27141944
- Full Text :
- https://doi.org/10.1111/j.1774-7909.2007.00575.x