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Protective effect of sodium butyrate on the cell culture model of Huntington disease.
- Source :
-
Progress in Natural Science . Jul2007, Vol. 17 Issue 7, p784-788. 5p. 1 Diagram, 1 Chart, 1 Graph. - Publication Year :
- 2007
-
Abstract
- This study aimed to develop a cell culture model of Huntington disease and observe the effect of sodium butyrate on this cell culture model. Exon 1 of both a wild type and a mutant IT15 gene from the genomic DNA of a healthy adult and a patient with Huntington disease was amplified and cloned into the eukaryotic expression vector pEGFP-C1. Human neuroblastoma SH-SY5Y cells were transiently transfected with these recombinant plasmids in the absence and presence of sodium butyrate (0. 1, 0. 2, 0. 5, 1 . 0 mmol/L). The MTT assay was used to measure cell viability. The results indicated that the N-terminal fragment of mutant huntingtin formed perinuclear and intranuclear aggregates and caused a decrease of SH-SY5Y cell viability. Sodium butyrate inhibited the decrease of SH-SY5Y cell viability caused by the N-terminal fragment of mutant huntingtin. This suggests that sodium butyrate has a protective effect on this cell culture model of Huntington disease. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL culture
*HUNTINGTON disease
*DNA
*GENETIC vectors
*SODIUM
Subjects
Details
- Language :
- English
- ISSN :
- 10020071
- Volume :
- 17
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Progress in Natural Science
- Publication Type :
- Academic Journal
- Accession number :
- 27013130
- Full Text :
- https://doi.org/10.1080/10002007088537473