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Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH
- Source :
-
Electrochemistry Communications . Sep2007, Vol. 9 Issue 9, p2240-2245. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at −0.6V. The biosensor showed a fast response to nitrite (5s) with a linear range between 0.015 and 2.35μM, a sensitivity of 1.8AM−1 cm−2 and a detection limit of 4nM. The apparent Michaelis–Menten constant () was 7.5μM. [Copyright &y& Elsevier]
- Subjects :
- *BIOSENSORS
*DETECTORS
*MEDICAL equipment
*HYDROXIDES
Subjects
Details
- Language :
- English
- ISSN :
- 13882481
- Volume :
- 9
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Electrochemistry Communications
- Publication Type :
- Academic Journal
- Accession number :
- 26337486
- Full Text :
- https://doi.org/10.1016/j.elecom.2007.05.030