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Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

Authors :
Chen, H.
Mousty, C.
Cosnier, S.
Silveira, C.
Moura, J.J.G.
Almeida, M.G.
Source :
Electrochemistry Communications. Sep2007, Vol. 9 Issue 9, p2240-2245. 6p.
Publication Year :
2007

Abstract

Abstract: A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at −0.6V. The biosensor showed a fast response to nitrite (5s) with a linear range between 0.015 and 2.35μM, a sensitivity of 1.8AM−1 cm−2 and a detection limit of 4nM. The apparent Michaelis–Menten constant () was 7.5μM. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
13882481
Volume :
9
Issue :
9
Database :
Academic Search Index
Journal :
Electrochemistry Communications
Publication Type :
Academic Journal
Accession number :
26337486
Full Text :
https://doi.org/10.1016/j.elecom.2007.05.030