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Characterization of the 3′ exonuclease of Chlamydophila pneumoniae endonuclease IV on double-stranded DNA and the RNA strand of RNA/DNA hybrid

Authors :
Liu, Xipeng
Zhang, Yang
Liang, Rubing
Hou, Jingli
Liu, Jianhua
Source :
Biochemical & Biophysical Research Communications. Oct2007, Vol. 361 Issue 4, p987-993. 7p.
Publication Year :
2007

Abstract

Abstract: Endonuclease IV has AP endonuclease and 3′-repair diesterase activities. Here, we report Chlamydophila pneumoniae endonuclease IV (CpEndoIV) could hydrolyze the ds DNA and the RNA strand of RNA/DNA hybrid from the 3′ end, yet the DNA strand of RNA/DNA hybrid was not the effective substrate of CpEndoIV. The optimal pH for 3′ exonuclease on double-stranded (ds) DNA and RNA/DNA hybrids were both basic, but with some difference. The effect of divalent ions (Mg2+, Ca2+, Zn2+, Cu2+, Ni2+, and Mn2+) on 3′ exonuclease was different for both substrates. High concentration of NaCl inhibited 3′ exonuclease on both substrates. For both substrates, the 3′ exonuclease activity of CpEndoIV on matched and mismatched 3′ end was comparable. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
0006291X
Volume :
361
Issue :
4
Database :
Academic Search Index
Journal :
Biochemical & Biophysical Research Communications
Publication Type :
Academic Journal
Accession number :
26246110
Full Text :
https://doi.org/10.1016/j.bbrc.2007.07.120